761 Contribution of resident immune cells in a human autologous 3D skin model

Skin wound healing results in hemostasis, inflammation, proliferation, and remodeling. The early phases require the recruitment of several immune cells from blood. However, role these is not well defined. In addition to monocytes-derived macrophages, known regulate outcome healing, resident macrophages injured skin may play a distinct process. aim was develop 3D autologous model containing endothelial cells, investigate their contribution during healing. A cell extraction technique firstly developed isolate same donor (keratinocytes, fibroblasts, cells). Analysis by flow cytometry revealed presence (CD45+ CD14+ CD163+); lymphocytes CD3+); dendritic CD14- CD1a+) (CD45- CD31+) freshly isolated cells. From this isolation, an vitro human developed. This approach applied 4 donors. Immunofluorescence staining confirmed previously described model. Endothelial self-assembled form capillary-like networks did exfiltrate after 14 days culture are able synthesize TNFα. Moreover, characterization proliferation rate epidermal basal layer Ki67 difference between reconstructed supplemented with compared only fibroblasts keratinocytes. These indicate that innovative provides great tool study interaction such as other wounded